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重组小鼠白介素-4(rmIL-4)

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CYT-282重组小鼠白介素-4(rmIL-4) 2μg/10μg/1mg Prospec

 

 

Description :
 Recombinant Mouse IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 13500 Dalton.

Recombinant IL-4 is purified by proprietary chromatographic techniques.

 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.

 
Formulation:
 Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

 
Solubility:
 It is recommended to reconstitute the lyophilized IL-4 in sterile 18Mω-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
 Lyophilized IL-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IL-4 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

 
Purity:
 Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
 The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Ile-His-Gly.

 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
 ProSpec's Interleukin-4 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant prolifiration of Murine HT-2 cells is less then 2 ng/ml, corresponding to a Specific Activity of 5x105 IU/mg.

 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of IL-4.

 
Protein content:
 Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard.

 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 

 


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